ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
PEACH LATENT MOSAIC VIROID: NUCLEOTIDE SEQUENCE OF AN ITALIAN ISOLATE, SENSITIVE DETECTION USING RT-PCR AND GEOGRAPHIC DISTRIBUTION |
| Authors: | A.M. Shamloul, A. Minafra, A. Hadidi, H.E. Waterworth, L. Giunchedi, E.K. Allam |
| Abstract:
An Italian isolate of peach latent mosaic viroid (PLMVd) was reverse transcribed and amplified from nucleic acid extracts of infected tissue then cloned. Nucleotide sequence analysis of the clone revealed that the viroid is 336 nucleotides, 95% homologous to the French isolate and contains the conserved sequence of the hammerhead structure which is involved in viroid self-cleavage. DNA primers were utilized for cDNA synthesis and PCR amplification of a full-length viroid DNA product from extracts of PLMVd-infected peach tissue. Amplified PLMVd cDNA hybridized to 32P-labeled PLMVd cRNA probe and was detected from extracts of infected peach fruit, leaf, and bark tissues but not from extracts of uninfected tissue or peach tissue infected with the agent of peach mosaic disease. RT-PCR assays of peach germplasm from several countries showed that PLMVd is distributed world-wide in peach tissues from Europe, Asia, North America, and South America. | |
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