ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
PEACH LATENT MOSAIC AND PEAR BLISTER CANKER VIROIDS: DETECTION BY MOLECULAR HYBRIDIZATION AND RELATIONSHIPS WITH SPECIFIC MALADIES AFFECTING PEACH AND PEAR TREES. |
| Authors: | S. Ambrós, G. Llácer, J.C. Desvignes, R. Flores |
| Abstract:
Peach latent mosaic and pear blister canker viroids (PLMVd and PBCVd respectively) have been recently described and characterized. The available molecular clones have allowed the design of a procedure that combines mild tissue extraction of nucleic acids with dot-blot hybridization under stringent conditions using 32P- or digoxigenin-labelled complementary RNA probes. This procedure leads to a rapid, sensitive and reliable detection of both viroids. We have used this approach to investigate whether PLMVd and PBCVd are involved in other peach and pear diseases apart from those initially reported. Cross-protect ion bioassays have shown that the peach yellow mosaic disease described in Japan is probably induced also by PLMVd. Analysis by molecular hybridization has confirmed this assumption. On the other hand this same approach has demonstrated that while PBCVd is the causal agent of the bark alteration observed in the pear indicator A20, it is not involved in other bark disorders observed in the pear cultivars Williams and Comice. | |
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