ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
THE DETECTION OF MLOS ASSOCIATED WITH PEAR DECLINE IN PEAR TREES AND PEAR PSYLLIDS BY POLYMERASE CHAIN REACTION |
| Authors: | D.L. Davies, D.J. Barbara, M.F. Clark |
| Abstract:
Polymerase chain reaction techniques using primers to sequences in the 16S RNA gene of pear decline MLOs have been used for the specific detection of MLOs associated with pear decline in pear trees and in the psyllid vector of the disease. No product was obtained with these primers from DNA prepared from healthy pear tissue or from periwinkle plants infected with one of several MLOs including aster yellows, clover phyllody, apple proliferation, plum leptonecrosis, apricot chlorotic leaf roll, Moliere's disease and elm yellows. A nested PCR technique using a preliminary amplification with primers to sequences located outside the fragment amplified by PD specific primers was the most consistent means of obtaining a product from psyllid DNA preparations, and was sufficiently in single insects. | |
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