ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
IN VITRO BEHAVIOUR OF DIFFERENT HOSTS INFECTED WITH DIFFERENT FRUIT TREE MYCOPLASMA-LIKE ORGANISMS |
| Authors: | M. Lansac, W. Jarausch, F. Dosba |
| Abstract:
Since 1985, a collection of non-culturable mycoplasma-like organisms (MLOs) infecting various fruit tree species have been maintained in their natural and experimental host plants propagated in vitro. Different isolates of Malus-affecting MLOs associated with apple proliferation were multiplied in Malus pumila rootstock MM106 and in the woody indicator Pyronia veitchii. Different isolates of Prunus-affecting MLOs associated with severe diseases like apricot chlorotic leaf roll, Molières disease and plum decline diseases were maintained in the Prunus marianna rootstock GF 8–1 or in the Prunus domestica cv. Enduke Tardicotes. Two fruit tree-MLO isolates which had been experimentally transmitted by dodder to Catharanthus roseus were also included in this study. Thus, MLO-diseased shoot tips of C. roseus were cultured. PCR amplification of MLO-specific DNA fragments was employed to confirm the presence of different MLO isolates in infected micropropagated plants. DAPI staining was used to estimate the concentration of MLOs in plants. The in vitro behaviour of different host plants infected with various MLO isolates was studied by analysing growth parameters, symptom expression and MLO concentration. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
