ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
NEW ASPECTS OF VIRUS ELIMINATION IN FRUIT TREES |
| Authors: | E. Knapp, V. Hanzer, H. Weiss H, A. da Câmara Machado, B. Weiss, Q. Wang, H. Katinger, M. Laimer da Câmara Machado |
| Abstract:
An in vitro virus elimination programme for Malus and Prunus species was established. Thermotherapy in vitro combined with meristem preparation was the method of choice. Malus was tested for ApMV, SGV and ACLSV, Prunus for PPV, PNRV, PDV, ApMV, and ACLSV by ELISA and by immuno tissue-printing. PPV, PNRV, SGV and ACLSV were detectable after 4 years of in vitro culture, PDV was found in a 8 month old Prunus culture. ApMV was lost after 2.5 years of in vitro culture. PPV could be detected in leaves after 20 days of thermotheraphy, but not after meristem culture from these shoots. SGV was either clearly positive after 33 days of thermotherapy or reduced below the threshold for reliable serological detection. SGV titre increased dramatically by six month after thermotherapy and meristem dissection. No ACLSV positives were found after 33 days of thermotherapy and shoots grown from meristems of main shoots were free of ACLSV six month after, whereas shoots from axillary meristems were infected. Nucleic acid based detection methods will be used for further evaluation of virus survival during thermotherapy. | |
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