ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
POSSIBILITY OF SHARKA (PLUM POX) VIRUS ISOLATION FROM PLUM AND APRICOT BRANCHES ACTIVATED DURING WITNER |
| Authors: | M. Babovic, A. Bulajic |
| Abstract:
Sharka (plum pox) virus, as an extremely significant plum and apricot virus in our country, has been studied more than most of the other fruit tree viruses. The diagnosis of this virus is, besides the possibility of using up-to-date serological methods (Rankovic and Vuksanovic, 1981; 1985), frequently preconditioned by visual observing and recording the symptoms. These problems are related to the sharka virus unbalanced distribution in the tissues of plum and apricot cultivars and to a low virus concentration during certain parts of the year. For the purpose of using serological methods in further studying of the virus, it is necessary to isolate and transfer the sharka virus to herbaceous indicator plants. In winter, during the dormancy period, it is possible to diagnose the sharka virus in the diseased plum and apricot trees (Roggero and Lenzi, 1985; Albrechtova, 1986), especially in the bark and dormant buds by ELISA test. This paper is an attempt to find the answer if it is possible to isolate the sharka virus from artificially forced branches during winter, and to transfer it to Chenopidum foetidum Schrad as an appropriate herbaceous indicator plant (van Oosten, 1970). Sutic and Babovic (1969) and Rankovic and Jordovic (1970) worked on similar problems but had small or no success. | |
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