ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
DETECTION AND CHARACTERIZATION OF PLUM POX VIRUS (PPV) ISOLATES FROM SOUTHERN GERMANY BY POLYMERASE CHAIN REACTION (PCR) |
| Authors: | G. Krczal, U. Avenarius |
| Abstract:
Plum pox virus (PPV) isolates show quantitative and qualitative differences in host plants, symptomatology, aphid transmissibility and molecular biology. In order to gain an overview on the different PPV strains that exist in Germany, several PPV isolates, mainly deriving from Southern Germany were analyzed by polymerase chain reaction (PCR), the reaction yielding a 243 bp product. All PPV isolates tested were amplified and the amplified fragments were analyzed by restriction endonuclease digestion. A RsaI restriction site polymorphism in the amplified fragments allowed the discrimination of two groups of isolates. | |
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