The aim of our studies was the isolation and characterization of apple chlorotic leaf spot virus (ACLSV) strains occurring in fruit trees in Poland, preparation of antisera and elaborating methods for routine ACLSV diagnosis in fruit trees.
ACLSV was detected in apple, pear, sweet cherry, plum and quince, using indirect F(ab')2 ELISA. Six isolates of ACLSV obtained from apple, pear, plum and sweet cherry were studied and antisera were prepared against five of them.
Plum isolate - SX/2 showed significantly weaker reaction in ELISA with two antisera (prepared against apple and cherry isolates) than other isolates did.
Another isolate - JPom, obtained from apple, reacted better with monoclonal antibodies EMA32 than the others did.
The coat protein (CP) of SX/2 isolate migrated faster in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) than CP of any other of the studied isolates.
Reverse transcription - polymerase chain reaction (RT-PCR) technique was applied to the studied isolated using samples of both herbaceous and woody hosts.
Specific products were obtained for all the isolates, except SX/2 and digested with three restriction enzymes.
Two different patterns were found for four isolates.
Pear fruits were good source of ACLSV for ELISA tests.