ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
CHARACTERIZATION AND DETECTION OF PLUM POX VIRUS USING MONOCLONAL ANTIBODIES |
| Authors: | M. Asensio, M.T. Gorris, E. Camarasa, E. Pérez, E.A. Carbonell, M. Cambra, A. Sanz |
| Abstract:
Eighty-five representative PPV isolates of different hosts and geographical origins were tested by ELISA-Double Antibody Sandwich Indirect (DASI), using eleven monoclonal antibodies (MCAs) produced against Spanish PPV isolates by hybridoma technology. Nine different epitopes were found and twenty three distinct serological patterns of reaction or serogroups were established using nine selected MCAs against the collection, showing a high variability in the coat protein among PPV isolates. Changes in epitope composition were observed in some PPV isolates after mechanical or aphid transmission and/or propagation in certain hosts, indicating the presence of natural mixtures of isolates in field trees. The ELISA-DASI data were analyzed using x2 distance. A dendrogram was obtained by the Lance and Williams procedure as the aggregation criteria, showing the relathionships between the PPV isolates tested. Two main clusters show a good separation between D and M PPV types. MCAs 4DG5 and 4DG11 only reacted against D types by ELISA-DASI and immunoprinting-ELISA, allowing a good differentiation. The MCA 5B was able to react with all PPV isolates assayed with high affinity. This antibody probably represents a well conserved epitope in the coat protein of PPV. The use of MCAs improved the specificity of serological techniques for detection and comparison of PPV isolates and provided a good tool for characterizing and differentiating PPV isolates. | |
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