ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
PATHOGEN-DERIVED RESISTANCE INDUCED BY INTEGRATING THE PLUM POX VIRUS COAT PROTEIN GENE INTO PLANTS OF NICOTIANA BENTHAMIANA |
| Authors: | L. Palkovics, A. Wittner, E. Balázs |
| Abstract:
The coat protein (CP) gene from a Hungarian isolate of plum pox virus (PPV-SK68) was cloned into a binary vector and introduced into Nicotiana benthamiana L. plants via Agrobacterium tumefaciens. The integration of the CP gene was confirmed using polymerase chain reaction. The transcription and expression of the integrated CP gene were detected by Northern and Western blots. Its effect on resistance to PPV was investigated by inoculation of the RI progeny of the transformed lines with purified PPV-SK68 virions. Six plant lines were tested; two of them were completely protected and four not protected at all. The resistance of the transformed lines and the attenuation or absence of symptom development are discussed. | |
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