ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
BIOSAFETY CONSIDERATION OF DIFFERENT PLUM POX POTYVIRUS (PPV) GENES USED FOR TRANSFORMATION OF PLANTS |
| Authors: | A. Korte, I. Kramer, E. Maiss, R. Casper |
| Abstract:
The transfer of pathogen derived genes into plants for establishing resistance has become of great interest for the control of various diseases affecting agronomical and horticultural important crops. Resistance to viral pathogenes has been achieved by introduction of different coat protein genes from numerous viruses into plants. Meanwhile additional virus genes have been integrated into plant genomes and successfully tested for their ability to confer resistance. Before release of transgenic plants in the field, risk assessment of genetically modified organisms and their interactions with the environment has to be performed. The use of viral genes in transgenic plants directs the attention to phenomena like heterologous encapsidation and recombination. To circumvent or minimize these properties of different PPV genes in respect to such events, Nicotiana benthamiana plants have been transformed by Agrobacterium-mediated gene transfer with PPV genes coding for the P1 protein, the cylindrical inclusion body (C1, helicase), the nuclear inclusion body b (NIb, polymerase) and the coat protein (CP) of a non aphid transmissible isolate. In addition modified CP genes leading to non translatable mRNAs in plants have been transformed. To facilitate a rapid screening of transformed tissues the transferred DNA contains beside the viral gene the gus reporter and the notll antibiotic resistance gene. Transgenic plants expressing the coat protein gene of an aphid transmissible isolate (PPV-AT) were inoculated with different potyviruses. In all cases a various degree of PPV coat protein was detected in the newly formed virus particles by using polyclonal antibodies directed to PPV. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
