ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
POTENTIAL OF (RS)-DHPA FOR INHIBITION OF PLUM POX AND PRUNUS NECROTIC RING SPOT VIRUSES IN VITRO |
| Authors: | M. Mainardi, E. Triolo |
| Abstract:
The (RS)-DHPA antiviral effectiveness was evaluated against PPV and PNRSV in shoot tip cultures of Mr. S. 2/5 plum rootstock. The drug at 40 ug ml-1 had efficiently inhibited PPV: 47 % of treated explants resulted virus-free. Twenty and 80 ug ml-1 showed lower anti-PPV activity, with 17 and 27 % of negative explants respectively. (RS)-DHPA reduced the appearance of a systemic necrotic process observed only on explants infected with PPV. The obtained virus-free microplants never showed this alteration. The results of the applications of (RS)-DHPA on explants infected with PNRSV did not enable us to emphasize antiviral activity against this virus. Our results underline the possibility of obtaining PPV-free material usable for the subsequent propagation through application of (RS)-DHPA combined with the in vitro technique. | |
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