ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
BIOLOGICAL AND MOLECULAR BIOLOGICAL INVESTIGATIONS OF SEVERAL PLUM POX VIRUS (PPV) ISOLATES |
| Authors: | G. Deborré, W. Jelkman, E. Maiss |
| Abstract:
Several PPV strains were investigated for symptom expression in herbaceous plants and for aphid transmissibility to different hosts. The coat protein sequence of these isolates was analyzed. The experiments included 7 greenhouse isolates, kept in Nicotiana clevelandii and 16 field isolates, kept in Prunus spp. Differences in symptom expression in herbaceous plants were classified. Aphid transmissibility was lost with some isolates maintained in tobacco. All aphid transmissible PPV isolates show a DAG amino acid motif close to the N-terminus of the coat protein, whereas the motif of non transmissible strains changed to DAL or NAG. Furthermore, an unusual feature was the deletion of 42 nt in the coat protein of one isolate. Maintenance of identical isolates at different locations for several years resulted in no mutations or a low mutation frequency. Two isolates lost aphid transmissibility after repeated mechanical inoculation. The coat protein sequences of 19 isolates were obtained. Computer restriction site analysis of these isolates and newly published PPV sequences, demonstrated the absence of a Rsa I site from oriental and some occidental strains. Strains that had no RsaI site are also missing a Dra I and Sfu I site, possibly indicating a close phylogenetic relationship among these isolates. | |
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