ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
DETECTION AND PARTIAL CHARACTERIZATION OF A PLUM POX VIRUS ISOLATE FROM INFECTED SOUR CHERRY |
| Authors: | L. Nemchinov, A. Hadidi, T. Verderevskaya |
| Abstract:
Characterization of the sour cherry isolate of plum pox virus (PPV) from Moldova (Kalashyan et al., 1989, 1994; Nemchinov et al., 1994) has been investigated by RT-PCR assays, molecular hybrdization and nucleotide sequencing analysis. A 220 bp DNA fragment from infected tissue was amplified using DNA primers for the 3' non-coding region (3' NCR) of PPV. The nucleotide sequence of this fragment is at least 95% homologous to that of other PPV isolates. RT-PCR assays of several sour cherry cultivars and molecular hybridization tests with 3' NCR PPV cRNA probe demonstrated that PPV is systemically distributed in infected trees. RFLP analysis of IC-RT-PCR amplified products using DNA primers for the carboxy terminal region of PPV coat protein showed that the sour cherry isolate of PPV is a unique member of the M strain or the prototype of a new strain which contains neither Rsa 1 nor Alu 1 restriction site. This information was confirmed by nucleotide sequence analysis of several clones of the amplified product from PPV-infected sour cherry tissue. | |
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