ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
PLUM POX VIRUS (PPV) IN SWEET CHERRY |
| Authors: | A. Crescenzi, M. Nuzzaci, P. Piazzolla, L. Levy, A. Hadidi |
| Abstract:
Plum pox virus (PPV) was previously the only potyvirus known to infect stone fruits. Recently, however, a new potyvirus, named Asian prunus latent virus (APLV), has been reported to infect Prunus spp. This virus reacts positively in ELISA with PPV antiserum from France, but negatively with PPV DNA primers specific for the 3' non-coding region (NCR) of PPV in reverse trascription polymerase chain reaction (RT-PCR) assay. Biological and serological testing suggested the possible presence of PPV in sweet cherry (Prunus avium L., cv Ferrovia) from southern Italy. To determine whether the virus in sweet cherry was PPV or APLV, we utilized the 3' NCR DNA primers specific for PPV to assay for its presence in extracts of sweet cherry leaves determined to be positive by ELISA. Uninfected cherry tissue and PPV-infected plum tissue were used as controls. RT-PCR amplified DNA products from infected sweet cherry and plum tissue were of the expected size, 220 bp. No amplified products were obtained from healthy control. The 3' NCR product from PPV infected sweet cherry was isolated from 5% polyacrylamide gels and directly cloned using the TA cloning system. cRNA probes generated using T7 RNA polymerase were PPV specific. Nucleotide sequencing revealed the clones to be a portion of PPV genome. These results indicate that the virus in sweet cherry is indeed an isolate of PPV, confirming the biological and immunological findings that reveal the identity of PPV in sweet cherry from southern Italy. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
