ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
APHIDS AS POTENTIAL VECTORS OF PLUM POX VIRUS: COMPARISON OF METHODS OF TESTING AND EPIDEMIOLOGICAL CONSEQUENCES |
| Authors: | G. Labonne, M. Yvon, J.B. Quiot, L. Avinent, G. Llacer |
| Abstract:
A simple method named "unrestricted probing" has been designed to determine if a given aphid species can be a vector of plum pox virus (PPV). The method consists in setting together in a cage (50 x 40 x 40 cm) several thousand aphids of a given species with herbaceous plants susceptible to PPV mixed with virus infected plants. Aphid colonies are located in a dark container which is introduced in the cage. There, the aphids are free to leave their host plant, walk out of the container and move and probe on the infected and healthy plants. The "unrestricted probing" method and several other methods used to transmit PPV were compared using several aphid species. The "unrestricted probing" method appeared to be the most sensitive one. Using this method over a large range of aphid species, we found new vectors of PPV (such as Rhopalosiphum padi, Aphis hederae) and we confirmed as vectors some species which were previrously found able to transmit PPV only once (Aphis gossypii, Aphis fabae). We also found that distinct clones of A. gossypii transmit PPV with significantly different efficiencies. These results suggest that it is necessary to reassess the role of the different aphid species in PPV epidemiology. | |
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