ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
SYMPTOMS OF PRUNUS NECROTIC RINGSPOT VIRUS ON MICROGRAFTED PRUNUS SERRULATA CV. SHIROFUGEN SHOOT CULTURES |
| Authors: | S. Zilkah, E. Faingersh, A. Rotbaum, S. Spiegel, A. Stein |
| Abstract:
Prunus serrulata cv. Shirofugen is used worldwide as a biological indicator for virus diseases of Prunus spp. Indexing is performed traditionally by graft inoculations onto fully-developed Shirofugen plants which are then observed over a long period of time for-symptoms. In the present study, in vitro indexing was attempted with micropropagated Shirofugen shoots and prunus necrotic ringspot virus (PNRSV)-inf ected shoot cultures of peach (cvs. Hermosa and Elberta) and sweet cherry (cv. 40 E 50). Micrografting was done in vitro in two ways: (a) Shoot cultures of the infected cultivars were used as 'rootstocks' onto which Shirofugen shoots were micrografted as ‘scions’. (b) Shirofugen serving as ‘rootstocks’ and cultivars as ‘scions’. Healthy cultivars were included for controls. Preliminary results showed that a typical symptomatic necrosis developed when Shirofugen was grafted as a ‘scion’ onto PNRSV-infected Elberta peach and 40 E 50 sweet cherry and did not develop with healthy controls. These results are preliminary. To develop a reliable in vitro indexing system, additional work aimed to improve graft survival and reduce non specific reactions is necessary. | |
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