ISHS Acta Horticulturae 386: XVI International Symposium on Fruit Tree Virus diseases
CHARACTERIZATION OF ITALIAN ISOLATES OF PEAR VEIN YELLOWS VIRUS |
| Authors: | M. Pilotti, F. Faggioli, M. Barba |
| Abstract:
Pear vein yellows (PVY) disease is widespread in Italian pear orchards where it causes severe economical damages. An elongated virus was transmitted to Pyrus communis A28 clone and to Nicotiana occidentalis from pear plants showing symptoms of vein yellowing and one declining apple plant. Partial purification of viral particles was achieved from N. occidentalis by adding urea to the extraction buffer and Triton-X-100 to the clarified sap. The use of urea and Triton X-100 avoided particle aggregation and improved the yield of partially purified virus. Measurements of virus particles showed two major peaks, at 700–750 nm and at 1350–1400 nm, respectively. Aggregates of virus particles were seen in ultrathin sections of N. occidentalis infected leaves. Positive serological reactions were obtained with apple stem pitting virus and plum pox virus antisera. | |
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