ISHS Acta Horticulturae 381: International Symposium on Natural Phenols in Plant Resistance
TOTAL PHENOLS AND PHYTOALEXINS ACCUMULATION IN COFFEE LEAVES INFECTED WITH HEMILEIA VASTATRIX BERK ET BR. AND PSEUDOMONAS SYRINGAE |
| Authors: | M. E.M. Guedes, Leonor E. Guerra-Guimaräes, A. M.S. Silva, J.&e.;A. Cavaleiro |
| Abstract:
Two years old plants of Coffea arabica L. were inoculated with the rust Hemileia vastatrix Berk et Br. or infiltrated with a bacterial suspension of Pseudomonas syringae. The presence of biocidal compounds in the extracts was assessed by a bioassay method, with a dense suspension of Cladosporium cucumerinum ELL. et Arth (Allen & Kúc, 1968). The characterization of phytoalexins was made mainly by high-field NMR spectroscopy and mass spectrometry. The total phenol content was determined in the extract obtained from the acetone powders and calculated as the quantity of chlorogenic acid present. | |
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