ISHS Acta Horticulturae 381: International Symposium on Natural Phenols in Plant Resistance
THE ACTIVITY OF PHENYLALANINE AMMONIA-LYASE IN APPLE LEAVES AFTER WOUNDING |
| Authors: | S. Fünfgelder, U. Mayr, D. Treutter, W. Feucht |
| Abstract:
A spectrophotometric method for measuring the activity of phenylalanine ammonia-lyase (PAL, E.C. 4.3.1.5) was adapted to apple leaves. The enzyme is characterized by optima of pH (8.2) and temperature (55°C) and showed its highest activity in the young leaves, levelling down to 20% from the first to the fourth leaf. Pressure treatment of the leaf discs enhanced the enzyme activity in the light with a peak after 24 hours. The fact that soluble phenols accumulated later in treated discs than in untreated ones is discussed with reference to metabolic and catabolic processes. | |
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