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| Author: | M.A.N. Ejiofor |
Abstract:
Pure cultures of some linamarin-hydrolyzing microorganisms, namely: Alcaligenes faecalis, Leuconostoc mesenteroides, and Saccharomyces cerevisiae were prepared and used to inoculate fresh cassava pulp and left to ferment at 28–30°C for 96 h.
Their growth behavior in the cyanide-containing pulp (285 mg CN/kg) was observed.
All the microbes grew uninhibited, with generation times of 59, 58, and 78 min, respectively.
They hydrolyzed endogenous cassava linamarin, releasing 90, 94, and 102 mg CN/kg of pulp, respectively, whereas the cassava linamarase alone released only 58 mg CN/kg of pulp.
Further detoxication of the pulp was achieved when these organisms were inoculated singly or mixed on the fresh pulp.
This experiment was aimed at studying the possibility of producing minimum-cyanide gari, fufu or any other fermented cassava-based product.
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