ISHS Acta Horticulturae 380: Symposium on Tropical Root Crops in a Developping Economy
CARBON SOURCE REQUIREMENT FOR THE CULTURE OF WHITE YAM (DIOSCOREA ROTUNDATA) EMBRYOS IN VITRO |
| Authors: | C.E.A. Okezie, S.N.C. Okonkwo, F.I.O. Nwoke |
| Abstract:
Twelve carbohydrates (glucose, sucrose, fructose, galactose, mannose, lactose, maltose, raffinose, ribose, sorbose, xylose, and starch) were screened for the best carbon source for in vitro culture of white guinea yam (Dioscorea rotundata) embryos using Linsmaier/Skoog basal medium supplemented with a vitamin (thiamine hydrochloride) and myoinositol. Whole plantlets (complete with leaves, stem, and roots) were regenerated only when glucose or sucrose served as the carbon source, while roots and stunted shoot buds were produced under fructose, galactose, and mannose treatments within 12 wk of growth. Lactose, maltose, and raffinose supported only root production, while ribose, sorbose, xylose, and starch did not support any growth at all within the same period. Although there was no significant difference (P = 0.05) between the total growth (plantlet fresh weight) supported by glucose and by sucrose, more callus was formed under glucose treatments while sucrose treatments supported more shooting. The ability of sucrose to support more shooting than glucose became more apparent with increase in frequency of subculturing. In general, more uniform seedlings were produced under sucrose than under glucose treatments. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
