ISHS Acta Horticulturae 377: VIII International Symposium on Virus Diseases of Ornamental Plants
HYDRANGEA VIRESCENCE, A DISEASE ASSOCIATED WITH MYCOPLASMALIKE ORGANISMS IN CANADA |
| Authors: | C. Hiruki, X.D. Rong, S.J. Deng |
| Keywords: | disease diagnosis, mollicutes, MLO |
| Abstract:
A virescence disease of hydrangea (Hydrangea macrophylla) obtained from a local nursery was studied. Flower, petiole, and young stem tissues of both diseased and healthy plants were subjected to histochemical and electron microscopic examination. Pleomorphic bodies of mycoplasmalike organisms (MLO) were detected in the phloem elements of the diseased specimens, but not in healthy plants. Fluorescence microscopy revealed a DNA-specific staining with DAPI (4',6-diamidino-2-phenylindole, 2HCl). In polymerase chain reaction (PCR) amplification of DNA using two primer pairs synthesized according to the sequence of pCP88 containing clover proliferation (CP) MLO DNA, hydrangea virescence (HV) MLO DNA did not show hybridization signals. Using PCR primers for amplifications of different regions of 16S rRNA gene of mollicutes, no amplification was obtained with HV MLO-infected plants, while the amplifications were positive with potato witches'-broom and CP-MLO infected plants. | |
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