ISHS Acta Horticulturae 370: International Symposium on Tropical Fruits
IDENTIFICATION OF CASHEW (ANACARDIUM OCCIDENTALE L.) SEEDLINGS WITH RAPD MARKERS |
| Authors: | S.P. Silva Neto, I. Maruta, F. Takaiwa, K. Oono, K. Matsumoto |
| Keywords: | Random amplified polymorphic DNA, PCR, DNA Markers, cashew, Anacardium occidentale |
| Abstract:
An evaluation was made of the use of random amplified polymorphic DNA (RAPD) as a genetic fingerprinting system in cashew. Amplification products were obtained in genomic DNA extracted from seedlings of 4 dwarf cashew clones (CP06, CP09, CP76 and CP1001) amplified with six 10-mer arbitrary primers. The profiles obtained for each seedling were compared to each other for identification of clone specific amplified products. The size of the DNA fragments ranged from 240 to 1780 base pairs. The number of bands in the profiles varied from zero to 4, depending on the primer and seedling tested. A total of 27 amplification products that could be used for DNA fingerprinting were disclosed. Only the bands generated by one of the six primers tested were sufficient to distinguish each one of the four clone seedlings included in this study. Two primers could be selectec to be used in further studies for the identification of RAPD genomic markers in cashew. The results obtained revels that the use of RAPD technique is feasible for germoplasm analysis and characterization in cashew. | |
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