ISHS Acta Horticulturae 370: International Symposium on Tropical Fruits
IN VITRO GROWTH OF ENCAPSULATED SHOOT TIPS IN BANANA (MUSA SP) |
| Authors: | K. Matsumoto, C. Hirao, J. Teixeira |
| Abstract:
In order to establish a large-scale and low cost propagation method of banana (Musa sp), the synthetic-seed construction was attempted using in vitro propagated multiple shoots. The synthetic seeds, i.e. encapsulated propagules by calcium alginate gel, were constructed by immersing the shoot tips into 3% sodium alginate solution and dropping them into 68 mM calcium chloride solution. Nutrient components and plant growth regulators were added to the synthetic seeds during the encapsulation and the seeds were transplanted on liquid media for germination. When the capsules contained a medium with 6% or higher concentrations of sucrose, synthetic seeds grew on the germination medium without sucrose. However, when the capsules had the medium with only 4% or lower concentrations, the sucrose in germination medium was indispensable to make the synthetic seeds grow. After one month of conservation (25 °C, dark), all synthetic seeds developed shoots, when both the encapsulation medium and the germination medium were supplemented with 2% sucrose. Although further studies are needed, these results indicate the possibility of synthetic seed construction in banana. | |
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