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ISHS Acta Horticulturae 368: International Symposium on Postharvest Treatment of Horticultural Crops

CHLOROPHYLLASE BEHAVIOUR ON GRANNY SMITH APPLES

Authors:   M. Ihl, C. Etcheberrigaray, V. Bifani
Keywords:   chlorophyll, chlorophyllide, malus pumula, ripening, postharvest
Abstract:
Granny Smith apples, harvested mature, were stored in refrigerated conditions (RC) at 0–1°C, 90 – 95 % RH or in ambient conditions (AC) at 7–18°C, 80–90 % RH. Changes in fruit weight, soluble solids, pH, firmness, chlorophyll a,b and chlorophyllide a,b were followed during storage. Chlorophyllase enzyme was extracted from the exocarp and measured as nmoles chlorophyllide a,b/g fresh matter (FM). On unripe apples, the enzyme was inactive. While apples ripen on the tree, chlorophyllase becomes more active. During 133 days of storage at RC, enzyme activity remains high while at AC, it is very low.

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