ISHS Acta Horticulturae 367: VI International Symposium on Pear Growing
IN VITRO ROOTING OF QUINCE A (CYDONIA OBLONGA MILL.) |
| Authors: | H. Dumanoglu, Y. Gulsen |
| Abstract:
Effects of different auxin treatments on in vitro rooting of micropropagated shoots of East Malling Quince A (Cydonia oblonga Mill.) were investigated. In the first experiment IBA, IAA and NAA at 0.0, 0.1, 0.5 and 1.0 mg/l concentrations were added to solidified Murashige and Skoog (1962) basal medium with full strength macroelements. NAA was found to be the most effective auxin with 1.0 mg/l giving the highest rooting rate (26.7%). The second experiment was established to compare the addition of NAA at concentrations of 0.0, 0.5, 1.0 and 1.5 mg/l in the medium with the rapid immersion of microcuttings into 0, 500, 1000 and 2000 mg/l IBA solutions for 5 seconds and 1 minute. In both experiments microcuttings were cultured in MS medium containing 1/1 and ½ macroelements for 30 days. The effects of treatments on rooting rate, average root number and root length, percentage of root dry matter, and callus were determined. Rooting was best in Murashige and Skoog medium with half strength macroelements after a 5 sec. (90.9% rooting) or 1 min. (83.3% rooting) dip of the base of the microcutting into 2000 mg/l IBA. | |
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