ISHS Acta Horticulturae 359: V International Symposium on Plum and Prune Genetic, Breeding and Pomology
CULTIVAR IDENTIFICATION IN PRUNUS DOMESTICA USING RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS |
| Authors: | D. Gregor, W. Hartmann, R. Stösser |
| Abstract:
The DNA polymorphism assay developed by Williams et al. (1990) was applied to Prunus domestica. This random amplified polymorphic DNA (RAPD) technique detects naturally occurring DNA polymorphisms using the polymerase chain reaction (PCR) in combination with a single short oligonucleotide primer of arbitrary sequence for the amplification of genome-specific "fingerprints". The amplification conditions were adapted to the use with Prunus domestica DNA, and a total of 145 primers was tested. The size of the amplification products ranged from 250 to 2600 basepairs (bp), with an average of 9 bands per primer/cultivar combination. Thirty-seven out of 145 primers revealed evaluable polymorphism among a set of ten arbitrarily chosen plum cultivars. Six primers were found that each detected enough genetic variation to allow complete differentiation of all the cultivars tested. The reliability of this method was tested by comparing DNA from various plants of the same cultivar as well as different methods of sample storage and preparation. | |
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