ISHS Acta Horticulturae 359: V International Symposium on Plum and Prune Genetic, Breeding and Pomology
PRELIMINARY STUDIES ON REGENERATION AND TRANSFORMATION OF PLUM (PRUNUS DOMESTICA L.) |
| Author: | S.D. Yancheva |
| Abstract:
In vitro regeneration of two plum genotypes was investigated starting from leaf explants. As optimum regeneration medium was established MS basal medium with addition of 2 % sucrose, 0,5 μM/l IBA and 7,5 μM/l TDZ. Shoots were produced in 46,25 % for cv. Stanley and 31,43 % for cv. Kjustendilska sliva. By histochemicaly assaying GUS activity transient transformation frequencies were established respectively for Agrobacterium tumefaciens strains EHA101 - 54 %, C58C1 - 46 %, Aglo - 34 %. These experiments are the basic prerequisite for the application of Agrobacterium mediated PPV coat protein gene transfer. | |
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