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| Authors: | H. Swartz, S. Kristine Naess, Z. Yongping, J. Cummaragunta, L. Luchsinger, Chris S. Walsh, H. Stiles, B. A. Turk, I. Fordham, R. H. Zimmerman, Joseph A. Fiola, B. Smith, J. Popenoe |
| Keywords: | genetic engineering, mutagenesis, organogenesis, ploidy |
Abstract:
The Maryland/Virginia/New Jersey/Wisconsin Rubus breeding program is attempting to provide cultivars capable of withstanding the temperature extremes (-30°C to +40°C) of a humid continental climate.
The use of various species and a wide range of cultivars is providing adapted selections.
Further progress is being made through the use of tissue culture-assisted ploidy manipulation and genetic engineering.
Critical to these protocols have been techniques to regenerate shoots from leaves.
Recent advances have consistently resulted in over 90% successful regeneration from the apical-most leaflets.
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