ISHS Acta Horticulturae 352: VI International Symposium on Rubus and Ribes
RESTRICTION SITE MAPPING OF RIBES NUCLEAR RIBOSOMAL DNA. |
| Authors: | W. Messinger, A. Liston, K. Hummer |
| Abstract:
A 2475 base pair region of the nuclear ribosomal repeat was amplified using the polymerase chain reaction. This region includes the entire 18s and 5.8s subunits, both internal transcribed spacers (ITS-1 and ITS-2), and 25 base pairs of the 26s coding region. The length of ITS-1, ITS-2, and the 5.8s gene was estimated at 675 base pairs, with 1800 base pairs remaining for the 18s gene. Restriction sites were mapped in 29 species of Ribes representing all sections. Most variable sites mapped to ITS-1. Among the species sampled, the black currants (section Coreosma) showed high levels of divergence. The three sections of gooseberries (Hesperia, Lobbia, and Grossularia) were united by several mutations. The ornamental currants (Calobotrya) also shared mutations. Sites of potential phylogenetic information generally crossed subgeneric boundaries and showed high levels of homoplasy. This data will be compared to a chloroplast phylogeny generated by similar methods in order to further illuminate the evolutionary history of this group. | |
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