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| Authors: | M. Nyman, A. Wallin |
Abstract:
A transient B-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts.
Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms.
GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture.
Callus selected on 10 μg/ml hygromycin produced shoots which exhibited GUS-activity.
The transformed nature of the shoots obtained after selection was confirmed by DNA-analysis.
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