ISHS Acta Horticulturae 341: IV International Mango Symposium
GENETIC TRANSFORMATION OF MANGO |
| Authors: | H. Mathews, H.D. Wilde, R.E. Litz, H.Y. Wetzstein |
| Abstract:
Genetically transformed somatic embryos of two mango cultivars, 'Hindi' and 'Keitt', were obtained using two different engineered strains of Agrobacterium tumefaciens. In 'Keitt', a monoembryonic cultivar, segments of somatic embryos derived from zygotic embryo explants were co-cultivated with Agrobacterium tumefaciens strain C58Cl containing the plasmid pGV 3850::1103 conferring resistance to the antibiotic kanamycin. After screening on selection medium, proliferating globular somatic embryos were transferred to maturation medium where they developed into plantlets. Nucellar proembryos of 'Hindi', a polyembryonic cultivar, were co-cultivated with Agrobacterium tumefaciens strain A208 harboring pTiT37-SE::PMON 9749 (9749 ASE) with marker genes encoding  -glucuronidase and neomycin phosphotransferase (NPT II). Transformed proembryos capable of growing on selection medium containing kanamycin stained blue in the presence of X-Gluc.
These proembryos were screened on solid medium with 400 mg/l kanamycin and then in liquid selection medium containing 100 mg/l kanamycin.
This stringent selection procedure enabled recovery of pure clones of transformed somatic embryos of 'Hindi'. Southern blot hybridization confirmed the integration of NPT II and GUS genes into the mango genome.
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