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ISHS Acta Horticulturae 341: IV International Mango Symposium

FREQUENCY AND CHARACTERISTICS OF ZYGOTIC SEEDLINGS FROM POLYEMBRYONIC MANGO CULTIVARS, DETERMINED USING ISOZYMES AS GENETIC MARKERS

Authors:   C. Degani, M. Cohen, O. Reuveni, R. El-Batsri, S. Gazit
Keywords:   Nucellar seedlings, apomixis, rootstock, Mangifera indica L.
Abstract:
Mango (Mangifera indica L.) seeds were produced by open-pollination of 'Turpentine' and '13–1', respectively, and by 'Turpentine' caged with '13–1'. Leaf extracts of the resultant seedlings were electrophoretically analyzed for isozymes of isocitrate dehydrogenase, phosphoglucose isomerase, phosphoglucomutase and triosephosphate isomerase. Based on this analysis, 22%, 20% and 13% of the seed progeny from open-pollinated '13–1' and 'Turpentine', and from 'Turpentine' caged with '13–1', respectively, contained zygotic embryos. Among the zygotic progeny of open-pollinated '13–1', 37% were identified as originating from outcrossing. Within each progeny population, the mean weight of zygotic seedlings was higher than that of nucellar seedlings. Seed weight was positively correlated with the number of seedlings per seed, as well as with their total weight. A significant relationship was found between seedling weight and sprouting order, with the heaviest being the first to sprout.

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