ISHS Acta Horticulturae 338: VI International Workshop on Fireblight
SCREENING FOR POTENTIAL ANTAGONISTS TO ERWINIA AMYLOVORA IN VITRO AND IN VIVO AMONG THE BACTERIAL MICROFLORA OF PEAR AND APPLE |
| Authors: | Peter G. Psallidas, A. Vitas, J. Tsiantos |
| Keywords: | Biological control, Pyrus amigdaliformis, Cotoneaster salicifolius |
| Abstract:
Two hundred and thirty bacterial isolates from the epiphytic microflora of different host plants were tested for antagonistic activity against Erwinia amylovora in vitro and in vivo. The isolates were obtained by plating the washings of different plant organs, from different locations at different time of the year, on NAS (Nutrient Agar + 5% Sucrose) and CCT (Ishimaru and Kloss, 1984) media. The antagonistic activity of the isolates against Erwinia amylovora was tested in vitro by the double-layer and filter paper methods. PYGA (Peptone 1.5%, Yest extract 0.3%, Glycerol 2% and Agar (purified) 0.8%), ASMA (Asparagine 0.2%, KH2PO4 0.5%, K2HPO4 0.2%, MgSO47H2O 0.02%, NaCl 0.05%, Mannitol 1% and Agar 1.5%) and ENA (Emerson - Nutrient Agar, Isenbeck and Schulz, 1985) were used as substrates. Eighty seven of the isolates exhibited antagonistic activity, at least in one medium by one method. Six isolates were selected and tested against Erwinia amylovora in vivo by inoculating, pear slices, Cotoneaster salicifolius tender shoots, and clusters of flowers of Pyrus amygdaliformis with a 1:1 mixture of pathogen : antagonist. All the isolates tested gave no satisfactory inhibition of Erwinia amylovora in vivo. | |
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