ISHS Acta Horticulturae 338: VI International Workshop on Fireblight
HOT WATER TREATMENT FOR ERADICATION OF ERWINIA AMYLOVORA FROM PROPAGATING WOOD |
| Authors: | H. Aldwinckle, H. Gustafson |
| Abstract:
The goal of this research is to develop a method for eradicating viable cells of Erwinia amylovora from symptomless scion budsticks of apple by immersion in hot water. The effect of duration of immersion in water at different temperatures on viability of dormant buds of cvs. "Idared" and "Empire" and on survival of E. amylovora were determined. Ca. 50% of buds of both cvs. survived 60 min at 48°C, but all buds were killed by 30 min at 50°C. Shoot tips of greenhouse - grown potted "Idared" and "Empire" trees were inoculated with E. amylovora. After lesions had ceased expanding, 1cm segments of the stem, directly below the lesion margin and including a node, were immersed in water at 48°C for 60 min. No viable E. amylovora was detected by direct plating of phosphate - buffer - ground tissue on CCT medium, or by plating of Kado 523 broth that had been inoculated with grindate and incubated for 18 hours. Unimmersed tissues yielded viable E. amylovora. A phytosanitary protocol for E. amylovora - free apple scions will be presented. | |
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