ISHS Acta Horticulturae 338: VI International Workshop on Fireblight
IDENTIFICATION AND CLONING OF ERWINIA HERBICOLA DNA RESPONSIBLE FOR SUPPRESION OF ERWINIA AMYLOVORA |
| Authors: | L.P. Kearns, H.K. Mahanty |
| Abstract:
TnphoA-insertion mutants which failed to inhibit Erwinia amylovora (inhibition-) were created from a naturally occurring inhibitory Erwinia herbicola strain. An 8 kb region common to three complementing cosmids was sub-cloned into pACYC184 to give recombinant plasmid, pAH8. pAH8 partially restored inhibitory activity to inhibition- mutants. Complementation by pAH8 was enhanced by the addition in trans of a 5 kb DNA fragment, not common to the three cosmids. Mini-cell protein analysis of the recombinant plasmids showed that the 8 kb region codes for two proteins, approximately 39 and 28 kd. At least one of these proteins must be essential for the production of the inhibitory activity against E. amylovora. Mutagenesis studies are being continued to define further the DNA locus involved and its gene product(s). | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
