ISHS Acta Horticulturae 338: VI International Workshop on Fireblight
METABOLITES FROM PSEUDOMONADS THAT INHIBIT THE GROWTH OF ERWINIA AMYLOVORA |
| Author: | R.E. Mitchell |
| Abstract:
Bacterial strains representative of a wide range of Pseudomonas species were screened on solid media for the production of compounds inhibiting the growth of Erwinia amylovora. Strains were selected from the International Collection of Microorganisms from Plants, and spotted onto agar plates and allowed to grow for 48 h. The secretion and diffusion of inhibitory compounds into the agar was tested by overlaying with log phase cultures of Erwinia amylovora, and incubation of the plates overnight at 26° to visualise the zones of no-growth. A wide range of inhibitory activities was observed across a range of species, suggesting that the pseudomonads are a good source of variation for chemical composition of inhibitors. An inhibitor from Pseudomonas glumae was purified and its structure was determined by NMR and mass spectrometry to be the tripeptide alanylhomoserinylaspartic acid. The amino acid components were determined to have the L-configuration by gas chromatography of their methyl ester N-trifluoroacetyl derivatives. | |
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