ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
GENERATING TETRAPLOID MELONS IN TISSUE CULTURE |
| Authors: | J. W. Adelberg, Bill B. Rhodes, Halina T. Skorupska |
| Abstract:
Tetraploid regenerants spontaneously occurred when melon (Cucumis melo L.) cotyledons were cultured on 10 μM benzyladenine (BA). These polyploids were easily screened by pollen morphometry from precocious male flowers formed at the first few nodes. Less than 50% of single bud-derived culture lines were mixoploid. Primary regenerants served as indicators of ploidy of the regenerants after 9 and 18 months of subculture. Thus, the origin of tetraploidy was prior to subculture. During this time, mixoploid cultures lost tetraploid members and shiftd toward diploidy. Nuclear DNA content of original explant tissue was 1.7 picograms (2C) without indications of 4C populations. The absence of cell divisions and tetraploid cells in the explant tissue points to early in vitro divisions as the source of the tetraploids. Regenerant plants were non-chimeral with respect to ploidy and many were quite fertile. Sterile triploid hybrids have been synthesized. | |
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