ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
SOMACLONAL VARIATION AS A TOOL FOR BREEDING TOMATO FOR RESISTANCE TO BACTERIAL CANKER |
| Authors: | R.W. van den Bulk, J.J.M. Dons |
| Keywords: | Clavibacter michiganensis subsp. michiganensis, in vitro selection, Lycopersicon esculentum, tissue culture |
| Abstract:
Disease-resistant plants can be obtained by th screening of tissue culture-derived plants or their progenies. The efficiency of selecting such plants may increase by applying in vitro selection. The perspectives of these approaches were investigated for the selection of tomato plants with resistance to bacterial canker (Clavibacter michiganensis subsp. michiganensis). Phytotoxic extracellular polysaccharides are produced by the pathogen, but appeared to be unsuitable for use as selective agent at the cellular level. Somaclones were regenerated from explants of the susceptible tomato cv. Moneymaker, and their progenies showed various phenotypic alterations. Since genetic, somaclonal variation was present, the progenies were tested for bacterial canker resistance with a fast screening method in the greenhouse. The evaluation of 279 progenies showed that some variation for severity of wilting was present. However, somaclones with a major increase in resistance level, and thus valuable for plant breeding, were not found. The results suggest that the potential of somaclonal variation as a source of resistance to bacterial canker is limited. | |
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