ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
THE INDUCTION AND MAINTENANCE OF CAULOGENESIS FROM UNDIFFERENTIATED CALLUS OF QUINCE (CYDONIA OBLONGA) |
| Author: | J.M. Chartier-Hollis |
| Abstract:
A basic methodology for the induction and maintenance of sustained plant regeneration from disorganized callus of Quince A (Cydonia oblonga) is described. Stem callus from axenic shoots on multiplication medium was transferred to MSP1 medium {MS Salts, 85 mM sucrose, 10.6 μM  -naphthalnacetic acid (NAA), 2025 μM 6-benzyladenine (BA), pH 5.81} and cultured at 25°C with a 16-h photoperiod, 35 μmol•m-2•s-1. Caulogenesis intially observed on this medium could not be sustained and a medium containing gibberellic acid (GA3) in addition to BA and indolebutyric acid (IBA) was required for continued adventitious shoot production.
Elongated shoots were rooted in vitro and plantlets were transferred to the glasshouse where they completed acclimatization.
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