ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
PLOIDY CHANGES IN "MITCHELL" PETUNIA |
| Authors: | K. Kamo, R. Griesbach |
| Keywords: | Petunia,somaclonal variation, protoplasts, haploid, chimeric plants |
| Abstract:
The haploid "Mitchell" petunia was propagated in the greenhouse and in tissue culture and the resulting plants checked for ploidy using three methods: chromosome counts of root tips, number of chloroplasts per guard cell pair, and microfluorometry. The majority (80%) of haploid plants propagated through tissue culture were chimeric rather than purely diploid or haploid as determined by chromosome counts or microfluorometry. Chimeric plants could not be detected using number of chloroplasts per guard cell pair. Protoplasts isolated from mesophyll cells of diploid plants were initially purely diploid, but increased levels of ploidy occurred in protoplasts at their first and second cell divisions. Auxin and cytokinin did not affect the ploidy changes at the initial cell divisions. Both tetraploid and diploid mesophyll protoplasts grew in culture at the same plating efficiencies. | |
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