ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
INCREASING BUD REGENERATION IN AN ORNAMENTAL CAMELLIA |
| Authors: | A. Tosca, R. Pandolfi |
| Keywords: | Calcium ionophore A23187, Camellia x williamsii, DMSO |
| Abstract:
The effect of the calcium ionophore A23187 dissolved in DMSO (dimethyl sulfoxide, 0.2% total volume) on the induction of adventitious buds in an ornamental camellia (Camellia x williamsii cv. Debbie) was investigated. Leaf and stem sections derived from in vitro or in vivo plants were cultured on MS medium supplemented with A23187 (0, 10, 20 μM) in callus induction medium [18 μM benzyladenine (BA), 10μM indolebutyric acid (IBA) only, in the regeneration medium (22.5 μM BA, 2.5 μM IBA) only, or in both. The number of regenerant calluses was enhanced both by A23187 and DMSO alone compared to the control. The number of buds per organogenic callus was highly increased by A23187 used both in callus induction and regeneration media. | |
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