ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
THE EFFECT OF MOTHER PLANT PRETREATMENT AND EXPLANT CHOICE ON REGENERATION FROM IN VITRO PEAR LEAVES |
| Authors: | E. Chevreau, C. Leblay |
| Abstract:
Adventitious bud regeneration has been obtained at high rates from in vitro leaves of two pear cultivars (Comice and Passe Crassane), but with a low level of reproducibility. The effect of various pretreatment of the mother cultures on leaf regeneration ability was tested. Horizontal position during subculturing produced numerous but shorter shoots, and induced a lower rate of regeneration from the leaves, as compared with vertical position. Among three cytokinins tested, benzyladenine (BA) at 2.22 μM induced the highest rate of multiplication and shoot elongation of the mother cultures, and the highest rate of regeneration from leaves. Reducing the interval between subcultures from 40 to 20 days decreased significantly the rate of regeneration. Leaves taken from the uppermost part of shoots had the highest rate of regeneration and produced the largest number of buds per regenerating explant. | |
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