ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
SOMATIC EMBRYOGENESIS IN SWEET POTATO IPOMOEA BATATAS (L.) LAM |
| Authors: | A. Sonnino, P. Mini |
| Keywords: | Propagation, 2,4-D, regeneration, genotypic dependence, tissue culture |
| Abstract:
Sweet potato plants of five clones (Q 23728, Q 23836, CN 1489-89, 288-6R W 190 F) were tested for their somatic embryogenesis capacity using callus derived from bud meristems of greenhouse-grown plants. Callus was induced using Murashige and Skoog medium with slightly modified organic constituents plus 2,4-dichlorophenoxyacetic acid (2,4-D). Callus was transferred to similar medium without 2,4-D to permit embryo formation. Somatic embryogenesis capability was found to be genotype-dependent. In the clone Q 23728, where this capability was high, clusters of fused and individual embryos at different stages of development were observed on 67% of calluses with a mean number of embryos per explant of 10.3; 483 completely developed plants were obtained. So far, in sweet potato, no evidence has been reported that somatic embryogenesis, induced through an intermediary callus phase, leads to genetic variability in the regenerated plants. Studies are now in progress to evaluate whether the regeneration process induces genetic variability. | |
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