ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
ORGANOGENESIS AND SOMATIC EMBRYOGENESIS |
| Author: | R. E. Litz |
| Abstract:
The ability to regenerate horticultural crops species efficiently from cell and tissue cultures is critical for the applications of many biotechnology procedures. For the most part, generation protocols have been determined empirically. The recent adoption of descriptive terms from animal development has stimulated a better understanding of morphogenesis from cell and tissue cultures. Cells in tissue cultures are viewed as first acquiring competence which is associated with altered differential gene regulation and expression. The commitment of competent cells for morphogenesis is affected by many factors, i.e., directive induction, including a complex interaction between genotype, the explant (and its stage of development), medium, etc. Morphogenesis is triggered, usually after of a new cells are subcultured onto a less complex medium, allowing the expression of a new developmental potential. This trigger is referred to as permissive induction. | |
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