ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
EFFICIENT SHOOT REGENERATION FROM LEAF SECTIONS OF HIGHBUSH BLUEBERRY SUITABLE FOR USE IN AGROBACTERIUM-MEDIATED TRANSFORMATIONS |
| Authors: | Lisa J. Rowland, E. L. Ogden |
| Keywords: | Vaccinium corymbosum, zeatin riboside, cytokinin |
| Abstract:
In our studies aimed at producing transgenic blueberry plants, we have shown that highbush blueberry is susceptible to infection by at least three commonly used Agrobacterium tumefaciens strains. More recently, we have examined the relative efficiency of shoot regeneration from three different highbush cultivars and investigated ways of improving the efficiency of shoot regeneration. Effectiveness of tissue culture medium supplemented with zeatin riboside or zeatin has been compared to medium supplemented with 2-isopentenyladenine (2iP). Results indicated that zeatin riboside is more effective than either 2iP or zeatin in promoting shoot regeneration and that leaf sections of 'Sunrise' are particularly amenable to shoot regeneration. Furthermore, the effect of increasing concentrations of kanamycin (kan) on inhibition of shoot regeneration has also been determined. Currently, several attempts have been made to introduce the nptll (kan) gene into blueberry and shoots have been regenerated on medium containing kanamycin. To date, however, results from Southern blot analyses indicate plants have not yet been transformed successfully. | |
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