ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
TRANSGENIC COAT PROTEIN AND ANTISENSE RNA RESISTANCE TO BEAN YELLOW MOSAIC POTYVIRUS |
| Authors: | J. Hammond, K.K. Kamo |
| Abstract:
The coat protein (CP) gene of bean yellow mosaic virus (BYMV), and an antisense (AS) construct consisting of the 3' half of the CP gene, the complete 3' non-coding region, and a short poly-A tract were separately introduced to Nicotiana benthamiana leaf pieces by Agrobacterium-mediated transformation. Plants were regenerated and screened for the presence of the desired gene using the polymerase chain reaction (PCR) and (for CP plants) ELISA with monoclonal antibodies. Homozygous R2 populations were challenged by inoculation with various concentrations of BYMV and other potyviruses. At least two types of resistance were observed for both CP and AS plants; some transformants exhibited resistance to initial infection, and others became infected but showed resistance to replication or transport of BYMV. Some plants apparently recovered from infection in later-produced leaves, in which no virus could be detected by ELISA or bioassay. Neither BYMV CP nor AS plants showed significant resistance to either pepper mottle virus or turnip mosaic virus, distantly related potyviruses. | |
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