ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
INCORPORATION OF THE GUS GENE INTO ORCHIDS THROUGH EMBRYO ELECTROPHORESIS |
| Authors: | R.J. Griesbach, J. Hammond |
| Keywords: | Calanthe, transformation, transgenic plant |
| Abstract:
Pollen and the apical meristems of Calanthe orchid embryos were exposed to DNA under various treatments and conditions. The only treatment that produced transgenic plants was embryo electrophoresis. A pipette containing DNA suspended in agarose dissolved in acetate buffer was placed on the apical meristem of the embryo, while the opposite end of the embryo was in contact with a pipette containing only buffer. The optimal conditions for gene transfer were at 50 volts and 0.5 milliampere for 10 min. Under these conditions, 55% of the embryos survived the treatment and 57% of those that survived were transformed. | |
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