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ISHS Acta Horticulturae 333: WOCMAP I - Medicinal and Aromatic Plants Conference: part 1 of 4

SAPONINS FROM BONGARDIA CHRYSOGONUM (L.) SPACH.GROWING IN TURKEY

Authors:   K.H.C. Baser, G. Toker, B. Sener
Keywords:   Saponin, triterpenoid, Bongardia, Berberidaceae, Hederagenin, Oleanolic acid
Abstract:
Considering the medicinal and pharmacological importance of saponins, it was interesting to subject the plant Bongardia chrysogonum (L.)Spach. to chemical investigation. This work mainly concerned with the isolation and structure elucidation of saponins. The tubers of this plant were collected from the vicinities of Gaziantep (Turkey). The dried powder of tubers was extracted with ethanol at room temperature. The combined ethanolic extracts were evaporated at reduced pressure. The residue was dissolved in methanol and ether was added to yield yellow coloured precipitate of crude saponins. This mixture was chromatographed on a column then the fractions obtained were further purified by prep tlc and crystallization. After the analysis of saponins by FABMS (negative mode), the acid hydrolysis of pure saponins with methanolic HCl furnished a mixture of triterpenoid sapogenols and sugars. They are identified by using chromatographic and spectroscopic methods. Two-dimensional NMR measurements were also carried out to verify the chemical shift assignments. The crude saponin mixture of the tubers of B.chrysogonum on chromatographic separation afforded two minor compounds 1,2 in addition to four triterpenoids reported previously (Baser et al., 1990).
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